Accurate Diagnosis
Designed for the qualitative detection and identification of different phenotypes of A1AT in serum samples. 100% of concordance with another agarose gel isocelectric focusing and in agreement with the different phenotypes.
Alpha-1 Antitrypsin (A1AT) Phenotype Testing
The reference method for the Alpha-1 Antitrypsin (A1AT) phenotype testing.
How do we diagnose A1AT Deficiency?
The most common method to detect A1AT Deficiency is protein phenotyping using isoelectric focusing.
The HYDRAGEL 18 A1AT ISOFOCUSING kit used on the HYDRASYS 2 Scan Focusing instrument provides qualitative detection of the different phenotypes of the A1AT in human serum.
The semi-automated HYDRASYS 2 instrument enables migration, immunofixation and direct detection on agarose gels, to obtain A1AT profiles for qualitative analysis.
Phenotyping results in conjunction with clinical findings and other laboratory assays aid in the diagnosis of Alpha-1 Antitrypsin Deficiency.
Related Pathologies
Technologies used
Key benefits
Alpha-1 Antitrypsin (A1AT) Phenotype Testing, a clear and concise overview of the various phenotypes linked to a common genetic disorder that is often diagnosed late.
Efficient Method
Clear bands visualization for a reliable interpretation.
Fast Result
Simple step-by-step procedure without membrane transfer for 2.5 hours of turnaround time.
Testing is part of algorithms
HYDRAGEL 18 A1AT ISOFOCUSING is included in both screening and diagnostic algorithms to provide clinicians with phenotype of A1AT proteins secreted in blood circulation.
Detection limit of an Alpha-1 Antitrypsin’s isoform:
5 mg/dL
Main characteristics
Test principle
The HYDRAGEL 18 A1AT ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing (IEF) followed by immunofixation.
- After the protein separation according to their isoelectric point, the gel is directly incubated with a peroxidase labelled anti-A1AT antiserum with no need for a membrane blotting step.
- Then, the gel is processed in order to remove unbound proteins before the enzymatic reaction.
- Finally, the gel is visually evaluated.
Sample type
Human serum samples can be used with HYDRAGEL 18 A1AT ISOFOCUSING.
Performances
- 2 stages-protocol: Isoelectrofocalisation and Immunofixation for an optimized result.
- A1AT controls (MM, MZ and MS) are included in each series of analysis to quickly identify M, S and Z phenotypes.
Product details
10 gels of 18 tests.
Reagents
- HYDRAGEL 18 A1AT ISOFOCUSING – P/N: 4357
Accessories
- ANTI-A1AT - PER – P/N: 4746
Knowledge & Science
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