Semi-Automated Test
providing standardised and reproducible results.
How do we diagnose von Willebrand Disease?
von Willebrand disease (vWD) is a genetic bleeding disorder caused by missing or defective von Willebrand factor (vWF).
Since von Willebrand disease is complex, its diagnosis requires a comprehensive panel of tests to ensure accurate detection and proper subtype classification. Multimer analysis is a key component of this diagnostic workup, providing essential information to clinicians for optimal patient management.
HYDRAGEL von WILLEBRAND MULTIMERS kit is designed for the detection of abnormalities in the vWF multimers distribution related to a hemorrhagic diathesis. The test allows to assess the overall size distribution of von Willebrand factor multimers in human plasma using agarose gel electrophoresis and immunofixation, with the semi-automated HYDRASYS 2 SCAN FOCUSING instrument.
Related Pathologies
Technologies used
Key benefits
von Willebrand Multimer Analysis: a simplified, standardised gel method, delivering results within a single day.
Simplified Technique
making this test accessible to any laboratory.
Within-Day Results
compared to in house tests delivering results in 4-5 days.
Traceability of the Result
with densitometry-based interpretations.
Assay procedure of 6 hours.
Main characteristics
Test principle
HYDRAGEL von WILLEBRAND MULTIMERS allows separating small, intermediate, and large molecular weight multimers.
The assay is carried out in two stages:
- Sample pre-treatment followed by an electrophoresis done in SDS agarose gel to separate proteins according to their molecular weight.
- Direct immunofixation of multimers in the gel using anti-human vWF antibody followed by immunoenzyme staining to visualize the different multimers.
The semi-automated HYDRASYS 2 SCAN FOCUSING instrument performs all the steps needed to obtain gels ready for visual and densitometry-based interpretations.
Sample type
Plasma samples
Performances
- Excellent reproducibility
- All multimer sizes
- Densitometry helps for results interpretation
- Patient’s profile overlayed with a normal control
Product details
Ready-to-use gels and reagents: 10 gels of 5 or 11 tests.
- HYDRAGEL 5 von WILLEBRAND MULTIMERS kit – P/N: 4359 to be associated to von WILLEBRAND MULTIMERS VISUALIZATION KIT – P/N: 4747
- HYDRAGEL 11 von WILLEBRAND MULTIMERS kit – P/N: 4360 to be associated to von WILLEBRAND MULTIMERS VISUALIZATION KIT – P/N: 4747 x2
Knowledge & Science
We offer daily assistance for technical and scientific matters.
We aim to educate and share scientific and medical educational information with our customers all around the world.
Understand in 2 minutes how capillary electrophoresis works, and why it gives such high and clear resolution in lab testing.
von Willebrand Multimer Analysis test can be performed on the following instrument
Efficiency re-dIFined.
The compact and affordable automation of Capillary Electrophoresis.
The high throughput solution for newborn hemoglobin disorders screening.
Capillary Electrophoresis solution to meet your high capacity needs.
High Throughput - Smart Automation.
A concentrate of Sebia’s expertise in a high throughput instrument.
This section contains information intended for wide distribution and may therefore contain product details or information that is not available or valid in your country. Please contact your local Sebia representative. Information intended for healthcare professionals. Carefully read the instructions in the reagent package inserts and instrument manuals.